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Anthony D. DiNozzo Senior was a businessman, and the father of Tony DiNozzo. He specialized in art, and often traveled to Europe. Since he dealt with "some pretty shady folks", his son assumed that he might be a con artist rather than a legitimate businessman. Senior dismissed that accusation, claiming that he was "an entrepreneur".

He was married multiple times. His first wife was Tony's mother, while his last was her best friend and Tony's godmother.